The Human Embryonic Stem Cell Core Facility (HESCCF) is a medium-sized laboratory providing services to an increasing number of research users who pay recharge costs to the facility. The current number of frequent users is 30. Created in 2005, the HESCCF is a cornerstone of the UCSD institutional commitment to leadership in Regenerative Medicine. It fosters and promotes HESC research at UCSD and partner institutes of the Sanford Consortium for Regenerative Medicine. The facility 1) trains scientists in the basic methods to conduct HESC research, 2) facilitates HESC research for many investigators, both established and beginning scientists, 3) provides a 'safe haven' that is sheltered from any federal funding source thus allowing unimpeded HESC research, and 4) provides state-of-the-at technologies necessary to advance stem cell research. The facility sustains ongoing research projects and allows the proposal of new projects necessitating such facilities.

Under general supervision, the Stem Cell Research Associate undertakes research projects in collaboration with academic supervisors, making innovative contributions. The incumbent will operate and maintain two Fluorescence Activated Cell Sorters (FACS) and two bench top flow cytometers. Independently performs standard and advanced laboratory procedures with Human Embryonic Stem Cells. Performs cell culture laboratory procedures. Maintains and expand new hES cell lines for Core users. Evaluates new reagents and protocols and prepares detailed analysis of results. Conducts advanced experiments such as qPCR, fluorescent and confocal microscopy, gel imaging, and proliferation assays. Performs protocol troubleshooting to help researchers overcome scientific bottlenecks. Attends technical seminars to learn how to integrate emerging technologies into the Core. Performs other duties as assigned.

• Theoretical knowledge of molecular biology, cellular biology or related field generally acquired through an advanced degree or an equivalent combination of education and experience.

• Experience with Flow Cytometry technology.

• Working practical knowledge of molecular biology, DNA techniques and protein biochemistry.

• Working knowledge of sterile cell culture techniques.

• Experience using microscopy to perform experiments.

• Experience using mathematical skills to analyze and develop statistical data.

• Demonstrated ability to perform repetitive and specialized laboratory experimental procedures. Ability to follow experimental protocols. Ability to learn new procedures.

• Experience and working knowledge of sterile techniques and mammalian cell culture practices. Knowledge of numerous types of laboratory equipment related to uses, maintenance, and repair.

• Experience with various computer software, hardware, standard office equipment and lab equipment.

• Acute attention to detail. Demonstrated ability to be resourceful and capable of handling the changing needs of the research lab.

• Excellent multi-tasking and organization skills to conduct a variety of tasks in an extremely fast-paced environment.

• Demonstrated ability to work independently and as part of a large, diverse group.

• Control of standard English in written and spoken form. Demonstrated ability to communicate effectively in writing, electronically, and by spoken word with a variety of people from vendors to faculty.

• Good interpersonal communication skills that facilitate professional, effective, and courteous interaction with a diverse population including employees, faculty, researchers, and outside agencies. Proven ability to effectively present information and recommendations.

• Theoretical or practical knowledge of human or murine stem cell culture.

• Employment is subject to a criminal background check.

• Occasional overtime, including nights, weekends and holidays may be required.

• Must be able to work in environment where biological, chemical, and radiological hazards are present, and human embryonic stem cell and animals are used in research.

• Must be willing to work with viruses that are infectious to humans, including adeno-associated viruses and lentiviral vectors which are derived from human immunodeficiency virus (HIV).